|
KMID : 1094720120170050966
|
|
Biotechnology and Bioprocess Engineering
2012 Volume.17 No. 5 p.966 ~ p.971
|
|
|
Inhibitory effects of epigallocatechin gallate and its glucoside on the human intestinal maltase inhibition
|
|
Nguyen Thi Thanh Hanh
Jung Sun-Hwa
Lee Sun
Ryu Hwa-Ja
Kang Hee-Kyoung
Moon Young-Hwan
Kim Young-Min
Kimura Atsuo
Kim Do-Man
|
|
|
Abstract
|
|
|
|
Human intestinal maltase (HMA) is an α-glucosidase responsible for the hydrolysis of α-1,4-linkages from the non-reducing end of malto-oligosaccharides. HMA has become an important target in the treatment of type-2 diabetes. In this study, epigallocatechin gallate (EGCG) and EGCG glucoside (EGCG-G1) were identified as inhibitors of HMA by an in vitro assay with IC50 of 20 ± 1.0 and 31.5 ± 1.0 μM, respectively. A Lineweaver-Burk plot confirmed that EGCG and EGCG-G1 were competitive inhibitors of maltose substrate against HMA and inhibition kinetic constants (K i ) calculated from a Dixon plot were 5.93 ± 0.26 and 7.88 ± 0.57 μM, respectively. Both EGCG and EGCG-G1 bound to the active site of HMA with numerous hydrophobic and hydrogen bond interactions.
|
|
|
KEYWORD
|
|
|
alpha-glucosidase, human intestinal maltase, EGCG, EGCG glucoside, molecular docking
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
 
|